| 从早期胚胎多能干细胞生成的嵌合鼠 |
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| 引用本文： | 宋震涛 李秋棠. 从早期胚胎多能干细胞生成的嵌合鼠[J]. 遗传学报, 1993, 20(6): 499-503 |
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| 作者姓名： | 宋震涛 李秋棠 |
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| 作者单位： | 北京大学生物系 100871(宋震涛，李秋棠，尚克刚)，北京大学生物系 100871(吴鹤龄) |
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| 摘 要： | 本文利用囊胚注射法将小鼠胚胎多能干细胞－ＣＣＥ细胞注射到发育３天半的昆明和Ｃ３７ＢＬ／６Ｊ小鼠受体囊胚腔内，经假孕鼠借腹怀胎，获３只ＣＣＥ细胞毛色嵌合鼠。实验共注射胚胎６５４个，经培养其恢复成活率７３．８％，胚胎移植后，假母受孕率及产仔率分别为３２．９％和５３％。在所获３只嵌合鼠中，２只为ＣＣＥ－昆明毛色嵌合鼠，１只为ＣＣＥ－Ｃ３７ＢＬ／６Ｊ毛色嵌合鼠，这是国内首次利用胚胎多能干细胞获得嵌合鼠。为
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| 关 键 词： | 胚胎 干细胞 胚胎移植 嵌合鼠 |
Formation of Mouse Chimeras from Early Embryonic Pluripotential Stem Cell |
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| Song Zhentao Li Qiutang Shang Kegang Wu Heling. Formation of Mouse Chimeras from Early Embryonic Pluripotential Stem Cell[J]. Journal of Genetics and Genomics, 1993, 20(6): 499-503 |
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| Authors: | Song Zhentao Li Qiutang Shang Kegang Wu Heling |
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| Abstract: | Three chimeric mice were produced by injecting the embryonic stem cell-CCE cells into the cavity of 3.5 day host blastocysts from Kunming and C57BL/6J mice. By analyses of Glucose Phosphate Ismerase (GPI-1), the results show a contribution from CCE cells in heart tissue of female Kunming chimera. Breeding experiments demonstrated that all chimeric mice were fertile but none of 3 chimeras produced germ line transmission. |
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| Keywords: | Embryonic stem cell Blastocyst injection Embryo transfer Chi-meric mice |
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