Preparative labeling of proteins with [35S]methionine. |
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Authors: | L W Browder J Wilkes D I Rodenhiser |
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Affiliation: | Department of Biological Sciences, University of Calgary, Alberta, Canada. |
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Abstract: | The most common technique for preparative labeling of proteins with radioisotopes for experimental purposes utilizes 125I. This isotope has certain limitations, including the emission of gamma- and X-irradiation, the release of gaseous 125I2 from solutions of Na 125I, and the potential for concentration of 125I in thyroid glands. We have discovered a means for labeling proteins rapidly and simply with [35S]methionine. The technique is applicable to a wide variety of proteins. Antibodies labeled by our technique remain functional. |
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