Use of phosph-cellulose paper disks for the assay of histone acetyltransferase. |
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Authors: | K Horiuchi D Fujimoto |
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Institution: | Hamamatsu University School of Medicine, Hamamatsu, Japan |
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Abstract: | A modified method for the assay of histone acetyltransferase is presented. Previously reported methods depended upon the determination of the incorporation of radioactivity from 14C]acetyl coenzyme A into trichloroacetic acid (TCA)-precipitable material. However, as shown in this paper, 14C]acetylated histone cannot be precipitated quantitatively by TCA. In the method described in this paper, phospho-cellulose (P-cellulose) paper disks are used as an adsorbent for 14C]acetylated histone and 0.05 m carbonate buffer, pH 9.2, is used as a washing medium. This P-cellulose disk method allows more quantitative determination of 14C]acetylated histone than the TCA-precipitation methods. |
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