Galatosylation and sialylation of mammalian glycoproteins produced by baculovirus-madiated gene expression in insect cells |
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| Authors: | Eun-Young?Yun Tae-Won?Goo Sung-Wan?Kim Kwang-Ho?Choi Jae-Sam?Hwang Seok-Woo?Kang Email author" target="_blank">O-Yu?KwonEmail author |
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| Institution: | (1) Department of Agricultural Biology, National Institute of Agricultural Science and Technology, 441-100 RDA,Suwon, Korea;(2) Department of Anatomy, College of Medicine, Chungnam National University, 301-747 Taejon, Korea |
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| Abstract: | The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes required for mammalian-type N-glycan synthesis. This study describes the effective mammalian-like production of glycoproteins, such as β-1,4-galactosyltransferase and α-2,6-sialyltransferase, in the insect cell line Sf9.Revisions requested 13 April 2005; Revisions received 17 May 2005 |
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| Keywords: | β -1 4-galactosyltransferase glycoprotein insect cell Sf9 α -2 6-sialyltransferase |
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