Flavobacterium johnsoniae SprA is a cell surface protein involved in gliding motility |
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Authors: | Nelson Shawn S Glocka Padden P Agarwal Sarika Grimm David P McBride Mark J |
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Institution: | Department of Biological Sciences, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211, USA. |
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Abstract: | Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface. |
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