Strains of protoplasts of Entomophthora egressa in spruce budworm larvae |
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Authors: | Gary B Dunphy June M Chadwick |
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Institution: | Department of Microbiology and Immunology, Queen''s University at Kingston, Kingston, Ontario K7L 3N6, Canada |
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Abstract: | Protoplasts of strain 458 (S458) and strain 521 (S521) of Entomophthora egressa had LD50s of 620 and 8.8 cells/insect, respectively, for sixth-instar spruce budworm larvae. Both protoplast strains exhibited biphasic growth profiles with comparable growth rates in the larval hemocoel. The growth rates of the fungal strains increased as the total hemocyte counts declined. Hemocytopenia was greatest and most rapid in larvae containing S521 protoplasts. Protoplasts of S521 exposed to α-mannosidase and β-N-acetylglucosaminidase were as virulent as the control cells. β-Galactosidase reduced protoplast virulence. |
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Keywords: | spruce budworm larvae protoplasts hemocytes β-galactosidase virulence |
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