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Transgenic carnation plants obtained by Agrobacterium tumefaciens-mediated transformation of petal explants |
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| Authors: | Anne-Claire van Altvorst Herma Koehorst Jan de Jong Hans J. M. Dons |
| Affiliation: | (1) Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O.B.16, 6700AA Wageningen, The Netherlands;(2) Department of Ornamental Crops, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O.B.16, 6700AA Wageningen, The Netherlands |
| Abstract: | Transgenic carnation plants were obtained after infection of petal explants with the supervirulent Agrobacterium tumefaciens strain AGLO. Southern blot techniques confirmed the transgenic nature of four transformed plants. The expression of the gus gene was verified in these plants by histochemical assays on selected shoots. It was very difficult to transfer the transgenic plants to the greenhouse due to vitrification and premature flowering.Abbreviations BA 6-benzyladenine - GA3 gibberellic acid - gus- glucuronidase gene - MS Murashige and Skoog (1962) - NAA naphthaleneacetic acid - nptII neomycin phosphotransferase II gene - PCR polymerase chain reaction |
| Keywords: | hyperhydricity regeneration |
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