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Isolation and terminal sequence determination of the major rat brain myelin proteolipid P7 apoprotein
Authors:J L Nussbaum  J F Rouayrenc  P Mandel  J Jollès  P Jollès
Institution:Centre de Neurochimie du CNRS, and Institut de Chimie Biologique, Faculté de Médecine, 67085 Strasbourg Cedex, France;Laboratoire de Biochimie, Université de Paris VI, 96 Boulevard Raspail, 75272 Paris, France
Abstract:The major rat brain myelin proteolipid P7 apoprotein has been isolated in pure form by a preparative sodium dodecylsulphate gel electrophoresis system. Automated Edman degradation permitted the establishment of the N-terminal sequence up to the 20th amino acid. The C-terminal sequence was determined by the action of carboxypeptidase A.
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