Discrete intracellular Ca2+ pools coupled to two distinct Ca2+ influx pathways in human platelets |
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Authors: | Bing Sun Jun-ichi Kambayashi |
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Affiliation: | Vascular Biology, Maryland Research Laboratories, Otsuka America Pharmaceutical, Inc., Rockville, MD 20878, USA. bings@mrl.oapi.com |
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Abstract: | Ca(2+) influx is an important event associated with platelet activation and regulated by the content of intracellular Ca(2+). Previous studies have suggested two different Ca(2+) pools and two Ca(2+) influx pathways exist in platelets. In the present study, we have investigated the regulation of thrombin- and thapsigargin-induced Ca(2+) entry into human platelets, using fluorescent indicators to monitor Ca(2+) mobilization and membrane potential. It was found that depletion of thapsigargin-sensitive Ca(2+) stores was coupled to Ca(2+) influx through a Ca(2+)-selective pathway. Additional release of Ca(2+) from the thapsigargin-insensitive pool by thrombin caused the opening of a nonselective cation channel. |
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Keywords: | Thrombin Thapsigargin Inositol 1,4,5-trisphosphate Cation channel nonselective |
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