| 一种光学显微镜下观察原生质体的染色方法 |
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| 引用本文： | 杨世辉,方呈祥,张珞珍.一种光学显微镜下观察原生质体的染色方法[J].微生物学通报,2000,27(1):55-57. |
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| 作者姓名： | 杨世辉 方呈祥 张珞珍 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072 |
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| 摘 要： | Ｂｒｅｖｉｂａｃｔｅｒｉｕｍ ｌａｃｔｏｆｅｒｍｅｎｔｕｍ菌液经溶菌酶处理后分别与４种微生物染色液混合，在光学显微镜下比较观察原生质体形态的效果。结果表明；染色样品中的原生质体比未经染色的形态清晰易观察，而且显微照相效果好；其中使用草酸铵结晶紫和复红染色液的效果更佳。该方法程序简单、操作方便、效果明显，还适用于悬滴法观察菌体形态和细菌运动方式。
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| 关 键 词： | 原生质体 染色 草酸铵结晶紫 复红 |
| 文章编号： | 0253-2654(2000)-01-055-03 |
| 修稿时间： | 1998-10-12 |
A STAlNING METHOD USED FOR OBSERVING PROTOPLASTSUNDER THE LIGHT MICROSCOPE |
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| YANG Shi-Hui,FANG Cheng-Xiang,ZHANG Luo-Zhen.A STAlNING METHOD USED FOR OBSERVING PROTOPLASTSUNDER THE LIGHT MICROSCOPE[J].Microbiology,2000,27(1):55-57. |
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| Authors: | YANG Shi-Hui FANG Cheng-Xiang ZHANG Luo-Zhen |
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| Abstract: | Brevibacterium lactofermentum chltures digested by lysozyme were mixed with four dyes for microorganism staining, and then compared with observing effects of protoplasts under the light microscope. The results showed that the effect of the protoplasts was clearer, more convenient and better for photography in the specimens stained than that of unstained. The effects of ammonium oxalate-crystal violet and basic fuchsin dyes were better. This method has such superioties as simple procedure, convenient operation and obvious effect It can also be applied to observe the shape and the motion Pattern of the bacteria |
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| Keywords: | Protoplast Staining Ammonium oxalate-crystal violet Basic fuchin |
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