Spontaneous and flow-induced Ca2+ transients in retracted regions in endothelial cells |
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Authors: | Miyazaki T Ohata H Yamamoto M Momose K |
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Institution: | Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan. |
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Abstract: | Changes in intracellular calcium concentration (Ca2+]i) and focal adhesion sites of cultured bovine aortic endothelial cells (BAECs) were simultaneously visualized in real time. Local Ca2+]i transients were observed at the rear edges of spontaneously migrating BAECs. Furthermore, the majority of starting regions of Ca2+]i transients retracted continuously. Frequency of Ca2+]i transients increased with the application of fluid flow. The majority of starting regions of flow-induced Ca2+]i transients retracted following the occurrence of Ca2+]i transients. In addition, retracted areas were distributed in the upstream regions of the cell. Application of GdCl3, a mechanosensitive cation channel blocker, resulted in a clear reduction of Ca2+]i transients and rear retractions in cases of spontaneous and flow-induced BAEC migration. Flow-induced directional rear retractions were also inhibited. Consequently, we conclude that local Ca2+]i transients play an important role in the migration of BAECs with respect to rear retraction. Furthermore, flow-induced Ca2+]i transients regulate directional rear retraction under flow conditions. |
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