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Sequence analysis of genomic DNA (680 Mb) by GS-FLX-Titanium sequencer in the monogonont rotifer, Brachionus ibericus |
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| Authors: | Jae-Seong Lee Ryeo-Ok Kim Jae-Sung Rhee Jeonghoon Han Dae-Sik Hwang Beom-Soon Choi Chang Joo Lee Yong-Dal Yoon Jong-Sung Lim Young-Mi Lee Gyung Soo Park Atsushi Hagiwara Ik-Young Choi |
| Institution: | 1. National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea 2. Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul, 133-791, South Korea 3. National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, South Korea 7. Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea 4. Department of Green Life Science, College of Convergence, Sangmyung University, Seoul, 110-743, South Korea 5. Department of Marine Biotechnology, College of Liberal Arts and Sciences, Anyang University, Ganghwa, 417-833, South Korea 6. Graduate School of Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan |
| Abstract: | The monogonont rotifer, Brachionus ibericus (S type), is considered to be a promising model species for developmental biology, evolution, and environmental genomics. In an attempt to accelerate the molecular understanding of B. ibericus, we sequenced 680.5 Mb of genomic DNA using the genome sequencer GS-FLX-Titanium. We obtained 2,062,621 reads (average read length 329.9 bp) and 145,418 contigs (total contigs length 125.7 Mb) after excluding small reads (less than 200 bp) from the assembly, and finally obtained 10,133 unigenes (E value ?? 9.00E?04) after non-redundant (NR) BLAST search. In this article, we summarize the genomic DNA sequences of B. ibericus and discuss their potential use in the study of reproductive biology, endocrinology, environmental genomics, and ecotoxicological studies, and for providing insight into the genetic basis of mechanisms such as egg formation, antioxidant stress defense, and xenobiotic metabolism. |
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