Karyological and biochemical evidence for chromosomal dedifferentiation in neoplasia |
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Authors: | Raymond L. Teplitz Kenneth J. Barr Helen J. Lawce |
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Affiliation: | (1) Department of Cytogenetics and Cytology, City of Hope National Medical Center, 91010 Duarte, California |
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Abstract: | Summary Quantitative analysis of the X-linked enzyme, glucose 6-phosphate dehydrogenase (G-6-PD), was performed on tumor cells lines from two human females. Both tumor cells were hyperdiploid, having complete or redundant C+X groups. One, no. 930, lacked the X chromatin body and exhibited twice the level of G-6-PD as in the X chromatin-positive tumor cells, ME-180. Hence, in the no. 930 cell, reversal of X chromosomal condensation was associated with loss of the X chromatin body and doubling of genetic activity. Cells of no. 930 were subsequently placed in culture where after three passages they developed an X chromatin body (or bodies). G-6-PD determinations made at that time showed enzyme levels comparable to the X chromatin-positive tumor cells (ME-180). This research was supported by United States Public Health Service Grant CA 08791-03. |
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