New insights into bioprotective effectiveness of disaccharides: an FTIR study of human haemoglobin aqueous solutions exposed to static magnetic fields |
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Authors: | Salvatore Magazù Emanuele Calabrò Salvatore Campo Salvatore Interdonato |
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Institution: | (1) Department of Physics, University of Messina, Viale Stagno D’Alcontres, 31-98166 Messina, Italy;(2) Department of Biochemical, Physiological and Nutritional Sciences, University of Messina, Via C. Valeria, 98125 Messina, Italy |
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Abstract: | The aim of this study was the investigation of static magnetic field effects on haemoglobin secondary structure and the bioprotective
effectiveness of two disaccharides, sucrose and trehalose. Samples of haemoglobin aqueous solutions, in the absence and in
the presence of sucrose and trehalose, were exposed to a uniform magnetic field at 200 mT, which is the exposure limit established
by the ICNIRP recommendation for occupational exposure. Spectral analysis by FTIR spectroscopy after 3 and 7 h of exposure
revealed a decrease in the amide A vibration band for haemoglobin in bi-distilled water solution. Analogue exposures did not
produce any appreciable change of amide A for haemoglobin in sucrose and trehalose solutions. Otherwise, no relative increase
of
\upbeta \upbeta -sheet contents in amide I and II regions was detected for haemoglobin aqueous solutions, leading us to exclude the hypothesis
that static magnetic fields can induce the formation of aggregates in the protein. In addition, a decrease in CH3 stretching linkages occurred for haemoglobin in bi-distilled water solution after exposure, which was not observed for haemoglobin
in sucrose and trehalose aqueous solutions, providing further evidence of a bioprotective compensatory mechanism of such disaccharides. |
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Keywords: | Haemoglobin Static magnetic field Electromagnetic field Infrared spectroscopy Disaccharides Trehalose |
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