l-Myo-inositol-1-phosphate synthase from lower plant groups: Partial purification and properties of the enzyme from Euglena gracilis |
| |
Authors: | Sarmila Das Gupta Jukta Adhikari Arun Lahiri Majumder |
| |
Institution: | Biochemistry Lab, Dept of Botany, School of Life Sciences, Visva-bharati univ., Santiniketan 731235, India. |
| |
Abstract: | A survey for the enzyme L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) has been conducted among various members of the lower plant groups, mainly algac, bryophytes and fungi; some properties of the partially purified enzyme from Euglena gracilis Z . are presented. The enzyme was detected in Chloropycean algae, Marchantiales and the Basidiomycetous fungi. The enzyme from Euglena had a pH optimum at 7.5. The Km for glucose-6-P was 2.1 m M and for NAD+ 80 μ M . When assayed in the absence of added NAD+, the enzyme showed a basal activity suggesting the presence of bund NAD+ in the system. NH4Cl increased the enzyme activity two-fold, altough the enzyme was inactivated by (NH4)2SO4. |
| |
Keywords: | Inositol synthase myo-inositol |
|
|