Effects of pulsed electromagnetic field on intervertebral disc cell apoptosis in rats |
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| Authors: | Hamed Reihani Kermani Mehdi Pourghazi Saeed Esmaeili Mahani |
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| Institution: | 1. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences
KermanIran;2. Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman
KermanIran |
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| Abstract: | Despite numerous studies on pulsed electromagnetic field (PEMF) application, its effects of PEMF on intervertebral disc (IVD) have not yet been investigated in vivo. Accordingly, the effects of PEMF upon IVD in rats were evaluated through molecular surveys. Rats were divided into six groups: Group I and II were exposed to low and high frequency of PEMF (LF and HF, respectively). Group III and IV underwent induced disc degeneration and were exposed to low and high frequency of PEMF (LF/IDD and HF/IDD, respectively). Group V underwent induced disc degeneration (IDD), and group VI was control. The values of caspase 3, Bax, Bcl-2 and β-actin band density, as cell apoptotic markers, were obtained from band densitometry. Our results showed that the value of cleaved caspase-3 of cells and Bax/Bcl-2 ratio in IDD group increased significantly compared to the control group (p?p?
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| Keywords: | Apoptosis degeneration intervertebral disc pulsed electromagnetic field |
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