Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis |
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| Authors: | Mo Li Chunjing Bian |
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| Institution: | 1. Division of Molecular Medicine and Genetics;2. Department of Internal Medicine;3. University of Michigan Medical School;4. Ann Arbor, MI USA;5. These authors contributed equally to this work. |
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| Abstract: | Poly(ADP-ribosyl)ation is an unique posttranslational modification and required for spindle assembly and function during mitosis. However, the molecular mechanism of poly(ADP-ribose) (PAR) in mitosis remains elusive. Here, we show the evidence that PAR is recognized by ECT2, a key guanine nucleotide exchange factor in mitosis. The BRCT domain of ECT2 directly binds to PAR both in vitro and in vivo. We further found that α-tubulin is PARylated during mitosis. PARylation of α-tubulin is recognized by ECT2 and recruits ECT2 to mitotic spindle for completing mitosis. Taken together, our study reveals a novel mechanism by which PAR regulates mitosis. |
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| Keywords: | BRCT domain ECT2 mitosis PAR PAR binding |
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