Finding the best antibody dilution by repeated immunostaining of the same tissue section |
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| Authors: | AA Smith |
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| Affiliation: | Barry University School of Podiatric Medicine,Miami Shores, Florida |
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| Abstract: | One can find the optimal antibody dilution for immunostaining by repeated staining on the same tissue section by using a less dilute antibody for each attempt. Using secondary antibody and horseradish peroxidase conjugated to a dextran polymer, a section stained repeatedly with several dilutions of antibody appears as good as a section stained with only the last dilution. |
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| Keywords: | antibodies dilutions immunohistochemistry technique |
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