Effect of long-term 50 Hz magnetic field exposure on the micronucleated polychromatic erythrocytes of mice |
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Authors: | Miguel Alcaraz Encarnación Olmos Miguel Alcaraz-Saura Daniel G Achel Julián Castillo |
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Institution: | 1. Radiology and Physical Medicine Department, Faculty of Medicine, University of Murcia
MurciaSpain;2. Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission
Legon-AccraGhana |
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Abstract: | In recent years extremely low-frequency magnetic fields (ELF-EMF) have become widely used in human activities, leading to an increased chance of exposure to ELF-EMF. There are few reports on in vivo mammalian genotoxic effects using micronucleus (MN) assays, which generally have been used as a short-term screening system. We analyzed the possible genotoxic effect induced by long-term exposure (7, 14, 21, 28?d) of a 50?Hz ELM-MF to mice by measuring the increase in frequency of micronucleated polychromatic erythrocyte in their bone marrow (MNPCEs) and we compared it with that induced by 50?cGy of X-rays. Subsequently, we tried to reduce this chromosomal damage by administering four antioxidants substances with radioprotective capacities: dimethyl sulfoxide (DMSO), 6-n-propyl-2-thiouracil (PTU), grape-procyanidins (P) and citrus flavonoids extract (CE). The increase in micronucleated cells was higher in both physical treatments (Control?p?0.01) p?>?0.001)); however, the antioxidant substances only showed a genoprotective capacity against the damage induced by ionizing radiation (Ci?>?PTU?=?DMSO (p?0.001) >P?=?CE (p?0.001). The 50?Hz ELM-MF increased MNPCEs in mouse bone marrow, expressing a genotoxic capacity. Administration of antioxidant substances with radioprotective capacities known to act through the elimination of free radicals did not diminish the genotoxic effect induced by ELM-MF. |
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Keywords: | Antioxidants ELF magnetic fields genotoxic effects micronucleus radiation effects |
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