Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes |
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| Authors: | Ahmed Z Balboula Paula Stein |
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| Institution: | 1. Department of Genetics;2. Rutgers, The State University of New Jersey;3. Piscataway, NJ USA;4. Department of Biology;5. University of Pennsylvania;6. Philadelphia, PA USA;7. Theriogenology Department;8. Faculty of Veterinary Medicine;9. Mansoura University;10. Mansoura, Egypt;11. Department of Biology |
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| Abstract: | During mouse oocyte maturation histones are deacetylated, and inhibiting this deacetylation leads to abnormal chromosome segregation and aneuploidy. RBBP7 is a component of several different complexes that contain histone deacetylases, and therefore could be implicated in histone deacetylation. We find that Rbbp7 is a dormant maternal mRNA that is recruited for translation during oocyte maturation to regulate the histone deacetylation. Importantly, we show that the maturation-associated decrease of histone acetylation is required for localization and function of the chromosomal passenger complex (CPC) during oocyte meiotic maturation. This finding can explain the phenotypes of oocytes where Rbbp7 is depleted by an siRNA/morpholino cocktail including severe chromosome misalignment, improper kinetochore–microtubule attachments, impaired SAC function, cytokinesis defects, and increased incidence of aneuploidy at metaphase II (Met II). These results implicate RBBP7 as a novel regulator of histone deacetylation during oocyte maturation and provide evidence that such deacetylation is required for proper chromosome segregation by regulating localized CPC function. |
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| Keywords: | RBBP7 histone deacetylation Aurora kinase aneuploidy mouse oocyte CPC |
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