A sensitive spectrophotometric assay of pyruvate dehydrogenase activity |
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| Authors: | P W Scislowski E J Davis |
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| Affiliation: | 1. Laboratório de Morfologia Experimental, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Rua Universitária, 1619, Cascavel, Paraná, CEP: 85819-110, Brazil;2. Laboratório de Morfologia Experimental, Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Rua Universitária, 1619, Cascavel, Paraná, CEP: 85819-110, Brazil;3. Laboratório de Morfologia Experimental, Programa de Pós-Graduação em Biociências e Saúde, Universidade Estadual do Oeste do Paraná, Rua Universitária, 1619, Cascavel, Paraná, CEP: 85819-110, Brazil;4. Universidade Federal do Paraná, Rua General Rondon, 2195, Toledo, Paraná, CEP: 85902-090, Brazil;1. Programa de Pós-Graduação em Biociências e Saúde, UNIOESTE, Cascavel, Paraná, Brazil;2. Centro de Ciências Médicas e Farmacêuticas, UNIOESTE, Cascavel, Paraná, Brazil;3. Departamento de Anatomia, Instituto de Biociências, UNESP, Botucatu, São Paulo, Brazil;1. Physics Department, Ben Gurion University of the Negev, Beer Sheva, Israel;2. Physics Department, Oakland University, Rochester, MI, US;1. Fluids & Thermal Engineering Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK;2. Marine Engineering College, Dalian Maritime University, Dalian 116026, China;3. College of Mechanical and Electrical Engineering, Hohai University, Changzhou 213022, China;4. School of Physics, University of Nottingham, Nottingham NG7 2RD, UK;5. Fluids & Thermal Engineering Research Centre, University of Nottingham Ningbo, Ningbo 315100, China;1. Program in Conservation and Management of Natural Resources, Universidade Estadual do Oeste do Paraná, Rua Universitária, 2069, CEP 85819-110 Cascavel, Paraná, Brazil;2. Universidade Federal da Fronteira Sul, Rodovia BR 158, Km 405, CEP 85301-970 Laranjeiras do Sul, Paraná, Brazil;3. (Pecaa) Grupo de Pesquisas em Ciências Agro-Ambientais, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil |
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| Abstract: | The recently reported highly sensitive method for assay of acetyl-CoA:arylamine N-acetyltransferase (EC 2.3.1.5) [H. H. Andres, A. J. Klein, S. M. Szabo, and W. W. Weber (1985) 145, 367-375] has been adapted for determination of pyruvate dehydrogenase activity. This method provides an improvement in sensitivity over extant spectrophotometric methods and circumvents limitations of assays using radioactive pyruvate. In addition, the assay is simple and inexpensive and can be readily adapted for measurement of enzyme activity in crude tissue extracts or homogenates. |
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