Production ofBeauveria bassiana [Deuteromycotina: Hyphomycetes] in different liquid media and subsequent conidiation of dry mycelium |
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Authors: | M. C. Rombach R. M. Aguda D. W. Roberts |
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Affiliation: | (1) Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell, Tower Road, 14853 Ithaca, New York, U.S.A.;(2) Entomology Department, International Rice Research Institute, P.O. Box 933, Manila, the Philippines |
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Abstract: | Mycelium ofBeauveria bassiana can be grown in liquid culture, filtered, and the mycelium dried. After rehydration the mycelium sporulates. Two carbohydrate sources (sucrose and maltose), and one nitrogen/vitamin source (yeast extract) were tested for mycelium growth and subsequent conidial production. Maximum mycelium growth (12.31 mg/ml), in liquid culture, was in the sucrose (3.5%)/yeast extract (3.5%) medium, but mycelium from a maltose (2%)/yeast extract (0.75%) medium produced the maximum of 4.62×106 conidia/mg dry mycelium after incubation in moist Petri dishes. Using the data on mycelium yield (in liquid culture) and conidial production (by dry mycelium) it is calculated that the sucrose (3.5%)/yeast extract (3.5%) and the maltose (2%)/yeast extract (0.75%) media produce most conidia per media volume (an equivalent of 3.52–3.72×107 conidia/ml). |
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Keywords: | Beauveria bassiana marcescent process mass production fermenter dry mycelium conidiation |
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