Effect of Cephtriaxone and Cephtasidim Antibiotics on Enzymatic Hydrolysis of Chitosan-Based Pellicular Materials

Enzymatic hydrolysis of chitosan-based films supplemented with cephtasidim and cephtriaxone antibiotics on a substrate saturated with water, diluted acetic acid, or Ringer-Locke physiological saline was studied. Supplementation with antibiotics having the chemical structure of low molecular weight salts reduced the rate of enzymatic hydrolysis of chitosan regardless of the medium used. The decrease was related to the suppression of polyelectrolyte swelling of the polycation rather than enzyme inhibition. The addition of antibiotics to chitosan films can apparently be considered an approach to directed reduction of the film enzymatic hydrolysis rates, which may contribute to an increase in film lifetime on the wound surface.