Optimization of Metabolite Profiling for Black Medick (<Emphasis Type="Italic">Medicago lupulina</Emphasis>) and Peas (<Emphasis Type="Italic">Pisum sativum</Emphasis>)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Optimization of Metabolite Profiling for Black Medick (Medicago lupulina) and Peas (Pisum sativum)

Authors:	R. K. Puzanskiy V. V. Yemelyanov M. S. Kliukova A. L. Shavarda O. Yu. Shtark A. P. Yurkov M. F. Shishova

Affiliation:	1.St. Petersburg State University,St. Petersburg,Russia;2.Center for Molecular and Cell Technologies,St. Petersburg State University,St. Petersburg,Russia;3.All-Russia Research Institute for Agricultural Microbiology,St. Petersburg,Russia

Abstract:	Metabolic profiling is a key approach in current basic and applied research in biology. Comparative analysis of different metabolite extraction methods for pea (P. sativum) and black medick (M. lupulina) made it possible to find the optimal conditions for metabolite extraction and subsequent detection by gas chromatography coupled with mass spectrometry. The optimized method was shown to be reliable for assessment of the organ and species metabolic profiles for roots and leaves in pea and black medick plants.

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