Catalytic Domain of AfsKav Modulates Both Secondary Metabolism and Morphologic Differentiation in Streptomyces avermitilis ATCC 31272 |
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Authors: | A Rajkarnikar H-J Kwon Y-W Ryu J-W Suh |
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Institution: | (1) Department of Biologic Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin, 449-728, Korea;(2) Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Korea |
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Abstract: | Genetic characterization of afsK-av (SAV3816) in Streptomyces avermitilis ATCC 31272 was performed to evaluate the role(s) of this eukaryotic-type serine–threonine protein kinase (STPK) in the regulation
of morphologic differentiation and secondary metabolism. The afsK-av::neo mutant (SJW4001) was defective in sporulation, melanogenesis, and avermectin production. These phenotypic defects were complemented
by introduction of either the intact afsK-av or the 900-nt catalytic domain region. The catalytic domain restored sporulation and melanogenesis to SJW4001 whereas it
partially recovered avermectin production. This study reveals that AfsKav is a pleiotropic regulator and demonstrates in vivo
that the C-region of AfsKav is not essential for its regulatory role in S. avermitilis differentiations. |
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