High-performance liquid chromatographic assay of l-α-glycerophosphorylcholine using a two-step enzymic conversion |
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Authors: | G. Abbiati T. Fossati M. Arrigoni P. Rolle G.L. Dognini C. Castiglioni |
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Affiliation: | G. Abbiati*, T. Fossati, M. Arrigoni, P. Rolle, G.L. Dognini,C. Castiglioni |
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Abstract: | A high-performance liquid chromatographic method using an enzymic reactor for determination of l-α-glycerophosphorylcholine in pharmaceutical forms is described. The procedure includes incubation of l-α-glycerophosphorylcholine with glycerophosphorylcholine phosphodiesterase (EC 3.1.4.2), giving choline and glycerophosphate, and subsequent chromatography of choline with a post-column enzymic reactor and electrochemical detection. The results obtained show a close linearity of the whole assay from 2 to 150 nmol/ml l-α-glycerophosphorylcholine, the sensitivity being 2 pmol per 20 μl of injected sample. The precision of the method in the analysis of l-α-glycerophosphorylcholine in pharmaceutical forms, ampoules and capsules, was 1.34 and 1.21%, respectively. |
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