Catalysis of serine oligopeptidases is controlled by a gating filter mechanism |
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Authors: | Fülöp V Szeltner Z Polgár L |
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Affiliation: | Department of Biological Sciences, University of Warwick, Coventry, UK. |
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Abstract: | Proteases have a variety of strategies for selecting substrates in order to prevent uncontrolled protein degradation. A recent crystal structure determination of prolyl oligopeptidase has suggested a way for substrate selection involving an unclosed seven-bladed β-propeller domain. We have engineered a disulfide bond between the first and seventh blades of the propeller, which resulted in the loss of enzymatic activity. These results provided direct evidence for a novel strategy of regulation in which oscillating propeller blades act as a gating filter during catalysis, letting small peptide substrates into the active site while excluding large proteins to prevent accidental proteolysis. |
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