Interaction of pea (Pisum sativum L.) lectins with rhizobial strains |
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| Authors: | Manju Bajaj Giridhar Soni CK Singh |
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| Institution: | aDepartment of Biochemistry, Punjab Agricultural University, Ludhiana-141004, Punjab (India);bDepartment of Veterinary Pathology, Punjab Agricultural University, Ludhiana-141004, Punjab (India) |
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| Abstract: | Lectins from two varieties (PG-3 and LFP-48) of pea have been purified by affinity chromatography on Sephadex G-50. The specific activity increased by 23 and 25 folds, respectively. These lectins from both the varieties were found to be specific for mannose. The purified fluorescein isothiocyanate (FITC) – labelled lectins showed binding reaction with homologous as well as heterologous strains of Rhizobium spp. The results revealed that pea lectins are not highly specific to their respective rhizobia. Moreover, these lectins showed a greater stimulatory effect on homologous Rhizobium leguminosarum strains. |
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| Keywords: | Lectins Pea (Pisum sativum L ) Rhizobium |
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