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A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions |
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| Authors: | Lucas Gelain José Geraldo da Cruz Pradella Aline Carvalho da Costa Luuk van der Wielen Walter M. van Gulik |
| Affiliation: | 1. Delft University of Technology, Department of Biotechnology, Van der Maasweg 9, 2629HZ, Delft, the Netherlands;2. University of Campinas, School of Chemical Engineering, Av. Albert Einstein, 500, Campinas, Brazil;3. University of Limerick, Bernal Institute, V94 T9PX, Limerick, Ireland;4. Federal University of São Paulo, Institute of Science and Technology, Av. Cesare Mansueto Giulio Lattes, 1201, S. J. Campos, Brazil |
| Abstract: | Intracellular metabolites were evaluated during the continuous growth of Trichoderma harzianum P49P11 under carbon-limited conditions. Four different conditions in duplicate were investigated (10 and 20 g/L of glucose, 5.26/5.26 g/L of fructose/glucose and 10 g/L of sucrose in the feed). Differences in the values of some specific concentrations of intracellular metabolites were observed at steady-state for the duplicates. The presence of extracellular polysaccharide was confirmed in the supernatant of all conditions based on FT-IR and proton NMR. Fragments of polysaccharides from the cell wall could be released due to the shear stress and since the cells can consume them under carbon-limited conditions, this could create an unpredictable carbon flow rate into the cells. According to the values of the metabolite concentrations, it was considered that the consumption of those fragments was interfering with the analysis. |
| Keywords: | Continuous culture Carbon limitation Metabolites Fragments from extracellular polysaccharides |
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