High-affinity binding sites for the initiator protein DnaA on the chromosome of Escherichia coli |
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Authors: | Angelika Roth & Walter Messer |
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Institution: | Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany. |
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Abstract: | The initiator protein DnaA of Escherichia coli binds with unusually high affinity to five regions on the chromosome, in addition to the replication origin, oriC . Using a solid-phase DNA binding assay, in which the DNA binding C-terminal domain of DnaA is bound via a biotin tag to magnetic beads, we could fish only fragments with these six regions from different chromosomal digests. Except for oriC , these fragments contain only one or two consensus DnaA binding sites, DnaA boxes. The distribution of these high-affinity DnaA boxes on the chromosome is random. |
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