Laccase of the lignolytic fungus Trametes hirsuta: Purification and characterization of the enzyme, and cloning and primary structure of the gene |
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| Authors: | D N Rebrikov E V Stepanova O V Koroleva Zh I Budarina M V Zakharova T V Yurkova A S Solonin O V Belova Z A Pozhidaeva A A Leont’evsky |
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| Institution: | (1) Joint Stock Company DNA-Technology, Moscow, 115478, Russia;(2) Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia;(3) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moscow oblast, 142290, Russia |
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| Abstract: | The main physicochemical characteristics of the major isoform of the laccase secreted by the fungus Trametes hirsuta 072 were studied. The enzyme belongs to the group of high redox potential laccases (E T1 0 790 ± 5), and it oxidizes with high efficiency various substrates of phenolic nature. The gene of this isoform was cloned, and its nucleotide sequence was determined. The length of the complete gene is 2134 bp. It comprises 11 exons and 10 introns. Analysis of the amino acid sequence of T. hirsuta 072 laccase demonstrated a high homology to the other laccases secreted by fungi of the genus Trametes. |
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