| 丹参晚期胚胎富集蛋白基因SmLEA的表达特征研究 |
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| 引用本文： | 刘丛玲,王喆之.丹参晚期胚胎富集蛋白基因SmLEA的表达特征研究[J].中国生物工程杂志,2010,30(1):51-55. |
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| 作者姓名： | 刘丛玲 王喆之 |
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| 作者单位： | 陕西师范大学生命科学学院 药用植物资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室 西安 710062 |
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| 摘 要： | 利用DNA walking的方法克隆到了丹参SmLEA基因 5′ 端上游调控序列，约1 038bp。经生物信息学预测分析，该区域含有多种与逆境胁迫、ABA、种子特异表达相关的顺式作用元件。实时荧光定量PCR检测结果表明，100 μmol/L ABA，200mmol/L NaCl，低温（4℃）及脱水处理后，SmLEA基因的表达均得到了明显的提高，与启动子序列分析的结果相符合。表明SmLEA基因可对盐、脱水和低温胁迫以及 ABA做出响应。
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| 关 键 词： | 丹参 SmLEA基因 启动子 表达特征 |
| 收稿时间： | 2009-09-22 |
| 修稿时间： | 2009-11-05 |
Gene Expression Analysis of a Late Embryogenesis Abundant Gene from Salvia miltiorrhiza Bunge |
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| LIU Cong-ling,WANG Zhe-zhi.Gene Expression Analysis of a Late Embryogenesis Abundant Gene from Salvia miltiorrhiza Bunge[J].China Biotechnology,2010,30(1):51-55. |
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| Authors: | LIU Cong-ling WANG Zhe-zhi |
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| Institution: | Colledge of Life Scences, Shaanxi Normal University, Key Laboratory of Medicinal Plant Resources and Natural Pharmaceutical Chemistry,Ministry of Education，National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi’an 710062，China |
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| Abstract: | According to the sequence of SmLEA gene, a DNA fragment of 1 038 bp upstream of the coding sequence of SmLEA gene was amplified by DNA walking with the genomic DNA of Salvia miltiorrhiz as the template. Sequence analysis showed that the fragment contained some putative cis-elements relating to abiotic stress, ABA, seed specific expression. So the S. miltiorrhiz seedings was treated with 100μmol/L ABA, 200mmol/L NaCl,4℃, and subjected to dehydration. The real-time PCR showed that expression levels of SmLEA was increased obviously, which was in accordance with the sequence analysis. |
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| Keywords: | S miltiorrhiza SmLEA gene Promoter Expression pattern |
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