A Simplified Technique for Low Temperature Methyl Methacrylate Embedding |
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| Authors: | Chung-Ching Liu |
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| Institution: |
a Department of Medicine and Center for Research in Oral Biology, University of Washington, Seattle, Washington
b Department of American, Lake Veterans Administration Medical Center, Tacoma, Washington |
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| Abstract: | A simplified method for low temperature methyl methacrylate embedding with inhibited methyl methacrylate monomer is demonstrated using proper concentrations of benzoyl peroxide and N,N-dimethylaniline. The polymerized tissue blocks cut well and the tissue sections obtained show excellent acid phosphatase activity when demonstrated with the newly improved technique and Goldner's staining. Likewise, double tetracycline labels are well revealed by fluorescence microscopy. |
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