Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16 |
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Authors: | Thomas L. Freeman Michael J.D. San Francisco |
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Affiliation: | Department of Biological Sciences, Texas Tech University, Box 43131, Lubbock, TX 79409-3131, USA |
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Abstract: | Abstract A 3.4 kb fragment of Erwinia chrysanthemi EC16 DNA capable of complementing galacturonic acid uptake mutants ( exuT ) was identified and cloned into a multicopy vector. In E. chrysanthemi B374 exuT mutants, the cloned DNA provided for growth of the mutant strains on galacturonic acid by complementing the galacturonic acid uptake defect. Alkaline phosphatase ( phoA ) gene fusions with the cloned DNA suggested that most of the cloned DNA was necessary for complementation of exuT mutant strains. Using anti-alkaline phosphatase antibody, a hybrid ExuT-PhoA protein was localized to the membrane fraction of the bacterium. |
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Keywords: | Galacturonic acid transport Pectate lyase exuT Erwinia chrysanthemi |
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