Identification of essential genes in <Emphasis Type="Italic">C. jejuni</Emphasis> genome highlights hyper-variable plasticity regions |
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Authors: | Martin Stahl Alain Stintzi |
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Institution: | (1) Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, K1H 8M5, Canada; |
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Abstract: | A microarray transposon-based tracking approach was used to identify Campylobacter jejuni genes which are required for cell growth at 37°C, under a microaerophilic atmosphere and on a rich Mueller–Hinton medium.
A transposon-based mutant library, comprised of 7,201 individual mutants was constructed, representing 4.48× coverage of the
genome. An analysis of genes lacking a transposon insertion revealed 195 essential gene candidates. The function of these
genes represent many of the expected core functions of the cell, such as energy metabolism, macromolecule and cofactor biosynthesis,
cell structural proteins as well as basic cell processes. Forty-nine hypothetical proteins were also identified, further underlining
the importance of currently unknown proteins and pathways within C. jejuni. Unlike other bacteria, the essential genes were not uniformly distributed along the chromosome with three main regions lacking
essential genes. These particular regions corresponded to known hyper-variable plasticity regions of C. jejuni genome indicating, as expected, that these regions are dispensable in any given C. jejuni strain. Overall, this work identified dispensable and essential genes in C. jejuni that will ultimately lead to a better understanding of Campylobacter physiology. |
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