Methyl coenzyme M reductase (<Emphasis Type="Italic">mcrA</Emphasis>) gene based phylogenetic analysis of methanogens population in Murrah buffaloes (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>) |
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Authors: | Prem Prashant Chaudhary Sunil Kumar Sirohi Dheer Singh Jyoti Saxena |
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Institution: | (1) Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India; |
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Abstract: | The aim of the present study was to decipher the diversity of methanogens in rumen of Murrah buffaloes so that effective strategies
can be made in order to mitigate methane emission from these methanogens. In the present study diversity of rumen methanogens
in Murrah buffaloes (Bubalus bubalis) from North India was evaluated by using mcr-A gene library obtained from the pooled PCR product from four animals and by using MEGA4 software. A total of 104 clones were
examined, revealing 26 different mcr-A gene sequences or phylotypes. Of the 26 phylotypes, 16 (64 of 104 clones) were less than 97% similar to any of the cultured
strain of methanogens. Seven clone sequences were clustered with Methanomicrobium mobile and three clone sequences were clustered with Methanobrevibacter gottschalkii during the phylogenetic analysis. Uncultured group of methanogens comes out to be the major component of the methanogens
community structure in Murrah buffaloes. Methanomicrobium phylotype comes out to be major phylotype among cultured methanogens
followed by Methanobrevibacter phylotype. These results help in making effective strategies to check the growth of dominant
communities in the rumen of this animal which in turn help in the reduction of methane emission in the environment and ultimately
helps us in fighting with the problem of global warming. |
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