Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (<Emphasis Type="Italic">Passiflora edulis</Emphasis> Sims) genotypes |
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| Authors: | Daniela Lopes Paim Pinto Ana Maria Rocha de Almeida Mailson Monteiro Rêgo Maurecilne Lemes da Silva Evelyn Jardim de Oliveira Wagner Campos Otoni |
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| Institution: | 1.Laboratório de Cultura de Tecidos Vegetais/BIOAGRO, Departamento de Biologia Vegetal/BIOAGRO,Universidade Federal de Vi?osa,Vi?osa,Brazil;2.Department of Plant and Microbial Biology,University of California at Berkeley,Berkeley,USA;3.Centro de Ciências Agrárias,Universidade Federal da Paraíba,Areia,Brazil;4.Laboratório de Genética, Departamento de Ciências Biológicas,Universidade do Estado do Mato Grosso,Tangará da Serra,Brazil |
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| Abstract: | Mature zygotic embryos of three genotypes of Passiflora edulis Sims, including ‘FB-100’, ‘FB-200’, and ‘FB-300’ were incubated on a Murashige and Skoog (MS) (1962) medium supplemented with different concentrations (18.1–114.8 μM) of 2,4-diclorophenoxyacetic acid (2,4-D) and 4.4 μM of
6-benzyladenine (BA). MS basal medium and MS with BA induced germination of P. edulis embryos. The highest frequencies of embryogenic calli were observed when explants were incubated on MS medium supplemented
with 72.4 μM 2,4-D and 4.4 μM BA for ‘FB-200’, which showed the highest potential for embryogenic callus formation. Cytological
and histological analyses of pro-embryogenic callus revealed two distinct cell types: thin-walled, small, isodiametric cells
with large nuclei and dense cytoplasm, typical of intense metabolic activity; and elongated and vacuolated cells, with small
nuclei and less dense cytoplasm. Differentiation of somatic embryos was promoted on MS medium supplemented with activated
charcoal and indole-3-acetyl-l-aspartic acid (IAA-Asp) either with or without 2,4-D. However, no conversion of somatic embryos into plantlets was observed. |
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