Simple strategy for the in vitro conservation of Ipsea malabarica an endemic and endangered orchid of the Western Ghats of Kerala,India

A simple protocol was developed for plantlet regeneration from seedling leaf segments of pigeonpea cultivar ICPL 93115 through shoot organogenesis. Ten-day-old seedlings aseptically grown on MS medium were used for furnishing leaf segments. Initial incubation for 5 days in dark at 25±2 °C followed by transfer to 10/14-h light / dark cycle (12.1 mol photons m^–2 s^–1) favoured regeneration. The decisive role of 6-benzyl adenine at different concentrations was established on shoot organogenesis. Murashige and Skoog's (MS) (1962) medium fortified with BA of 5.0 mg l^–1 was optimum for shoot regeneration and MS + BA of 1.0 mg l^–1 for shoot elongation, while MS + IAA (1.0 mg l^–1) + kinetin (0.1 mg l^–1) showed good results for rooting.