Effect of Ammonia on Dark CO2 Fixation by Anabaena Cells Treated with Methionine Sulfoximine |
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Authors: | Ohmori Masayuki |
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Institution: | Laboratory of Marine Biochemistry, Ocean Research Institute, University of Tokyo Nakano-ku, Tokyo 164, Japan |
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Abstract: | Dark CO2 fixation by Anabaena cylindrica was stimulated aboutthree-fold by the addition of NH4Cl to the cells. The 14CO2incorporation experiments showed that 14C is most rapidly incorporatedinto aspartate and then glutamine by adding NH4CI. Glutamineaccumulated predominantly after the addition of NH4Cl showingthat NH4 is incorporated into glutamine by glutamine synthetase.The stimulating effect of NH4Cl on CO2 fixation and amino acidsynthesis was suppressed by methionine sulfoximine, an inhibitorof glutamine synthetase. It was suggested that dark CO2 fixationwas stimulated by the action of glutamine synthesis which isenhanced by ammonia. (Received February 10, 1981; Accepted April 2, 1981) |
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