DNA prime-protein boost based vaccination with a conserved region of
leptospiral immunoglobulin-like A and B proteins enhances protection against
leptospirosis |
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| Authors: | Karine M Forster Daiane D Hartwig Thaís L Oliveira Kátia L Bacelo Rodrigo Schuch Marta G Amaral Odir A Dellagostin |
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| Institution: | 1.Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Programa de Pós-Graduação em Biotecnologia, Pelotas, RS, Brasil;2.Universidade Federal de Pelotas, Instituto de Biologia, Departamento de Microbiologia e Parasitologia, Pelotas, RS, Brasil |
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| Abstract: | Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of
theLeptospira genus. Vaccination with bacterins has severe
limitations. Here, we evaluated the N-terminal region of the leptospiral
immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis
using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and
subunit vaccine. Upon challenge with a virulent strain ofLeptospira
interrogans, the prime-boost and DNA vaccine approaches induced
significant protection in hamsters, as well as a specific IgG antibody response and
sterilising immunity. Although vaccination with recombinant fragment of LigBrep also
produced a strong antibody response, it was not immunoprotective. These results
highlight the potential of LigBrep as a candidate antigen for an effective vaccine
against leptospirosis and emphasise the use of the DNA prime-protein boost as an
important strategy for vaccine development. |
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| Keywords: | leptospirosis Leptospira LigBrep subunit vaccine DNA vaccine prime-boost |
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