Anthelmintic effect of a water soluble Moringa oleifera lectin in rodents experimentally infected with Haemonchus contortus |
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| Institution: | 1. Departamento de Biociências, Centro de Ciências Biológicas e da Saúde, Universidade Federal Rural do Semi-Árido, Av. Francisco Mota, 572, Costa e Silva, 59625-900 Mossoró, Rio Grande do Norte, Brazil;2. Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Av. Professor Morais Rego, s/n, Cidade Universitária, 50670-901 Recife, Pernambuco, Brazil;1. Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo 00800, Sri Lanka;2. CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal 700032, India;3. Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Colombo 00800, Sri Lanka;1. JH-Department of Molecular Medicine, School of Interdisciplinary Sciences and Technology, Jamia Hamdard, New Delhi 110062, India;2. Centre for Research in Nanotechnology & Science, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India;3. Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration, Silver Spring, MD 20993, USA;4. National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, India;1. Laboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa, Ishikawa 920-1192, Japan;2. Department of Global Infectious Diseases, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan;3. Division of Gene Therapy, Jichi Medical University, Shimotsuke, Tochigi 329-0498, Japan;4. Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, Sulawesi Selatan 90245, Indonesia;5. Institute for Genetic Medicine, Hokkaido University, Sapporo, Hokkaido 060-0815, Japan |
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| Abstract: | Allied to the problem of gastrointestinal parasites, especially Haemochus contortus, the use of lectins of plant origin has contributed to the research of alternative anthelmintics. The nematicidal effect of a water soluble Moringa oleifera lectin (WSMoL) was investigated in an experimental model with H. contortus infected Wistar rodents. Three concentrations were tested orally: 5 mg/kg, 2.5 mg/kg and 1 mg/kg. The reduction in the number of larvae recovered in the experimental groups was analyzed, as well as biochemical, hematological and histological parameters. Treatments with 5, 2.5 and 1 mg/kg of WSMoL reduced the number of larvae recovered of animals by 74.7 %, 72.8 % and 66 %, respectively. Untreated infected animals had anemia, moderate mononuclear multifocal hepatitis, vascular congestion in the liver and kidneys, white pulp hyperplasia in the spleen, and presence of eosinophils in the intestine. Infected animals treated with 5 mg/kg of WSMoL showed liver with moderate bleeding, kidney with vascular congestion, spleen with white pulp hyperplasia and intestine with moderate presence of mononuclear cells. An increase in the serum level of glutamic pyruvic transaminase and a reduction in the level of hemoglobin (p < 0.001) were also observed in this group when compared to the uninfected group. However, the administered concentrations of 2.5 and 1 mg/kg of WSMoL were both satisfactory in terms of reducing the number of recovered larvae and not promoting negative changes in the biochemical, hematological and histological parameters evaluated. These results indicate an in vivo nematicidal effect of WSMoL on the H. contortus parasite. |
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