Colorimetric micromethods for glutaraldehyde determination by means of phenol and sulfuric acid or phenol and perchloric acid |
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| Authors: | J Boratyński T Zal |
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| Institution: | Department of Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw. |
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| Abstract: | Aqueous micromolar solutions of glutaraldehyde produce a yellow color when treated with 20% phenol in ethanol followed by concentrated sulfuric acid or with phenol in 70% perchloric acid. The absorbance at 482 or 479 nm, respectively, is linearly related to the glutaraldehyde concentration. Of the two methods developed, the sulfuric acid-phenol assay gives a higher sensitivity and the perchloric acid-phenol assay allows the determination of glutaraldehyde in the presence of sugars and proteins. |
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