BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei |
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| Authors: | Bowers Jolene R Engelthaler David M Ginther Jennifer L Pearson Talima Peacock Sharon J Tuanyok Apichai Wagner David M Currie Bart J Keim Paul S |
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| Institution: | Translational Genomics Research Institute, Flagstaff, Arizona, United States of America. jbowers@tgen.org |
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| Abstract: | A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. |
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