胰岛淀粉样多肽在豚鼠胰腺分布的免疫组织化学研究

本文用免疫组织化学ＡＢＣ法，研究了胰岛淀粉样多肽（Ｉｓｌｅｔａｍｙｌｏｉｄｐｏｌｙｐｅｐｔｉｄｅ，ＩＡＰＰ或称Ａｍｙｌｉｎ）在豚鼠胰脏的分布，并用邻片免疫组织化学双标记法，观察了ＩＡＰＰ与胰岛素（Ｉｎｓｕｌｉｎ，ＩＮＳ）、生长抑素（ＳｏｍａｔｏｓｔａｔｉｎＳＳ）的共存关系。结果显示，豚鼠胰岛内绝大多数细胞都呈ＩＡＰＰ阳性免疫反应，在胰外分泌部的腺泡和导管内也散在分布有ＩＡＰＰ免疫反应阳性细胞。多数ＩＡＰＰ免疫反应阳性的细胞都显示ＩＮＳ免疫反应阳性，胰岛内少数ＩＡＰＰ阳性细胞也呈ＳＳ免疫反应阳性。说明ＩＡＰＰ主要分布在豚鼠的胰岛内．但也少量存在于外分泌部。ＩＡＰＰ主要和ＩＮＳ共存于Ｂ细胞内。但也和ＳＳ共存于Ｄ细胞内，提示ＩＡＰＰ可能通过自分泌途径调节ＩＮＳ和ＳＳ的分泌。

IMMUNOHISTOCHEMICAL LOCALIZATION OF ISLET AMYLOID POLYPEPTIDE (IAPP) IN GUINEA PIG PANCRBAS

The distribution of islet Amyloid Polypeptide (IAPP) in pancreas of guinea pig was investigated with ABC immunostaining method. The coexistence of IAPP and insulin or somatostatin in the pancreas of guinea pig was examined with immunostaining of adjacent thin sections. The results indicated that the majority of pancreatic islet cells of guinea pig showed IAPP positive immunoreaction. A small number of IAPP immunoreactive'cells were scattered in the epithelia of acini and ducts of the pancreatic exocrine portion. Almost all IAPP positive immunoreactive cells also demonstrated insulin positive immunoreaction. Some IAPP positive immunoreactive cells in the islets showed somatostatin positive immunoreaction. These results suggested that the IAPP was mainly distributed in the pancreatic islets of guinea pig, but a smallpart of it was distributed in the pancreatic exocrine portion of guinea pig. The IAPP mainly coexisted in the B cells with insulin, and also coexisted in the D cells with somatostatin. Thus IAPP may regulate the secretion of the insulin and somatostatin by way of autocrine.