Preparative scale isolation of galactosylhydroxylysine. |
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Authors: | D F Smith |
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Affiliation: | The American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014 USA |
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Abstract: | A method is described for the isolation and purification of gram quantities of the hydroxylysine-monosaccharide from commercially available marine sponge. The procedure utilized alkaline hydrolysis followed by purification by ionexchange chromatography and gel filtration. Compositional analysis indicated that the final product contained only galactose, hydroxylysine, and HCl which were present in equimolar quantities and comprised 94% of the dry weight. This preparation has been utilized as a substrate for the assay of UDP-glucose:collagen glucosyltransferase (EC 2.4.1.66) of human platelets. |
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