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Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection
Authors:Chieko Taninaka  Hisakazu Ohtani  Erika Hanada  Hajime Kotaki  Hitoshi Sato  Tatsuji Iga
Institution:a Department of Pharmacy, the University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113-8655, Japan;b Department of Pharmacy, the Research Hospital, the Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-0071, Japan
Abstract:In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1–10 μg/ml for EM and RXM, and 0.03–3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9% and ±7%, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats.
Keywords:Erythromycin  Clarithromycin  Roxithromycin  Azithromycin
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