Pyruvate is transported by a proton symport inLactobacillus plantarum 8014 |
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Authors: | Jya-Li Tsau Arthur A Guffanti Dr Thomas J Montville |
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Institution: | (1) Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers-The State University, 08903 New Brunswick, New Jersey, USA;(2) Department of Biochemistry, Mount Sinai School of Medicine of the City University of New York, New York, New York, USA |
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Abstract: | Pyruvate is a key metabolic intermediate and the substrate for diacetyl and acetoin synthesis. The mechanism of pyruvate transport was determined inLactobacillus plantarum by use of cells and membrane vesicles. In the cells, protonophores inhibited pyruvate transport, whereas valinomycin did not. Pyruvate was accumulated against a gradient in membrane vesicles. The transport rate and the degree of accumulation increased as the proton gradient increased, but an imposed K potential of –61mV did not drive pyruvate transport. The maximum transport rate (35 nmol/min/mg protein) and accumulation ratio (162-fold) were at pH 3.0, with an apparent Km value of 35 M. These results suggested that pyruvate was transported by a proton symport. |
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