Two forms of NAD-dependent D-mandelate dehydrogenase in Enterococcus faecalis IAM 10071 |
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| Authors: | Tamura Yusuke Ohkubo Atsuko Iwai Saho Wada Yoichiro Shinoda Takeshi Arai Kazuhito Mineki Shigeru Iida Mitsugi Taguchi Hayao |
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| Affiliation: | Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan. |
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| Abstract: | Two forms of NAD-dependent D-mandelate dehydrogenase (D-ManDHs) were purified from Enterococcus faecalis IAM 10071. While these two enzymes consistently exhibited high activity toward large 2-ketoacid substrates that were branched at the C3 or C4 position, they gave distinctly different K(m) and V(max) values for these substrates and had distinct molecular weights by gel electrophoresis and gel filtration. |
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