Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos |
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Authors: | Stramer Brian Winfield Mark Shaw Tanya Millard Thomas H Woolner Sarah Martin Paul |
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Institution: | Department of Physiology, School of Medical Sciences, University of Bristol, UK. bstramer@rvc.ac.uk |
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Abstract: | By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection. |
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Keywords: | repair inflammation hemocyte antimicrobial GADD45 |
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