Supplementation of chilling storage medium with glutathione protects rooster sperm quality |
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| Affiliation: | 1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;3. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;4. Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran;5. Department of Clinical and Experimental Medicine (IKE), Division of Clinical Sciences, Obstetrics and Gynecology, Linköping University, Linköping, Sweden;6. Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain;1. Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand;2. Division of Animal Science, Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathum Thani, 12130, Thailand;3. Department of Animal Science, Yasothon College of Agriculture and Technology, Yasothon, 35130, Thailand;4. Department of Animal Science, Srisaket College of Agriculture and Technology, Srisaket, 33000, Thailand;5. School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand;6. Lumpayakang Deep Frozen Livestock Semen Production Center, Department of Livestock, Development, Ministry of Agriculture and Cooperatives, Village No. 2, Nong Ri Sub-district, Lam Sonthi District, Lop Buri, 15190, Thailand;1. Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;3. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Faculty of Veterinary Medicine, Department of Clinical Sciences, Shahid Chamran University, Ahvaz, Iran;3. Faculty of Agriculture, Department of Poultry Sciences, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | This study was aimed to evaluate the effect of addition of reduced glutathione (GSH) to the extender on the rooster's semen quality parameters and fertility potential. Semen samples were diluted with Lake extender contained 0, 0.5, 1, 2, 4 and 8 mM GSH. Then, were chilled to 5 °C and stored for a period of 48 h. Sperm motion characteristics, viability, membrane integrity, lipid peroxidation, mitochondrial activity and fertility potential were evaluated. At the initiation of the experiment (0 h), GSH did not affect sperm parameters, while 2–4 mM GSH improved (P ≤ 0.05) quality indicators during storage periods. Moreover, the samples treated with 2–4 mM GSH have had a lower lipid peroxidation compared to other groups (P ≤ 0.05). Artificial insemination using the semen samples, which had been stored in groups treated with 2–4 mM GSH for a period of 24 h, led to greater (P ≤ 0.05) fertilizing potential compared to the control group. |
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| Keywords: | Chilling Fertility Fresh semen GSH Rooster |
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